差异代谢物筛选

通过对代谢物进行筛选,寻找差异代谢物(biomarker)。相关差异代谢物筛选条件有:

  1. p-value ≤ 0.05 且 VIP ≥ 1[1]
  2. p-value ≤ 0.05 且 fold_change ≥ 1.5或≤0.667[2]
  3. one-way ANOVA p-value ≤ 0.05 且 VIP ≥ 1[3]
  4. two-way ANOVA p-value ≤ 0.05[4]
详细含义:

筛选值 筛选值含义 筛选阈值 备注
p-value 统计学上显著差异 p-value ≤ 0.05
VIP OPLS-DA第一主成分变量重要性值投影 VIP ≥ 1
Fold change 两两组别间倍性变化 fold_change ≥1.5或≤0.667 含量差异较大
one-way ANOVA p-value 一维方差分析 p-value ≤ 0.05 主要用于时间序列,梯度浓度处理等
two-way ANOVA p-value 两因素方差分析 p-value ≤ 0.05 一般为两因素情况,如对照-处理+时间序列样本
本实验采用条件 1 进行代谢物筛选,最终获得的差异代谢物见下表:

差异信息汇总信息
  dam_DEG.xlsx(正离子模式)
dam_DEG.xlsx 包含“stats”和“ALL_DEG.xlsx”两个工作簿,其中“stats”统计了各个比对组别差异代谢物的上、下调情况,“ALL_DEG”汇总了所有比对组别的差异代谢物信息。


mz rt rtmin VIP mean_Z sd_Z mean_M sd_M Fold Change_Z/M log2(FC_Z/M) p.value FDR
M415T806 415.214 805.937 13.430 1.571 5.206 5.416 0.000 0.000 85548.000 16.384 0.000 0.058
M309T92 309.130 91.729 1.530 2.399 1057.605 215.323 1708.095 198.497 0.619 -0.692 0.000 0.058
M566T834_2 566.331 834.046 13.900 2.175 174.919 46.462 368.277 114.120 0.475 -1.074 0.000 0.058
M310T92 310.133 91.726 1.530 2.444 397.147 66.799 660.402 91.110 0.601 -0.734 0.000 0.058
M104T775 104.108 774.682 12.910 1.063 23.648 11.839 866.802 1641.837 0.027 -5.196 0.000 0.058
M490T802 490.292 802.439 13.370 2.106 1144.249 225.351 741.724 120.744 1.543 0.625 0.000 0.058
M306T779 306.253 778.722 12.980 1.404 68.538 40.132 1833.903 2434.703 0.037 -4.742 0.000 0.058
M114T795 114.038 795.327 13.260 1.506 66.794 71.226 2.334 1.173 28.613 4.839 0.000 0.058
M192T90 192.025 90.107 1.500 2.163 51.719 19.415 14.970 10.088 3.455 1.789 0.000 0.058
M180T358 180.103 357.536 5.960 2.349 377.976 72.097 211.910 28.356 1.784 0.835 0.000 0.058
M141T71 140.996 70.909 1.180 1.546 2.199 1.969 87.315 102.721 0.025 -5.311 0.000 0.058
M313T104 313.018 103.938 1.730 2.172 1017.703 522.692 99.183 88.021 10.261 3.359 0.000 0.058
M173T140 172.976 139.936 2.330 2.011 1.639 1.120 42.776 31.898 0.038 -4.705 0.000 0.058
M112T777 111.523 777.174 12.950 1.291 11.877 15.799 0.218 0.616 54.478 5.768 0.001 0.058
M147T88 147.076 88.442 1.470 2.386 23.882 2.749 31.707 2.281 0.753 -0.409 0.001 0.058
M126T173 126.067 173.287 2.890 2.408 726.512 82.548 494.518 58.985 1.469 0.555 0.001 0.058
M127T321 127.051 321.207 5.350 2.165 26.591 6.836 12.559 5.002 2.117 1.082 0.001 0.058
M195T680 195.103 679.590 11.330 2.363 48.768 7.003 29.132 6.119 1.674 0.743 0.001 0.058
M525T737 525.387 736.737 12.280 2.110 7653.266 4664.611 55910.901 33321.421 0.137 -2.869 0.001 0.069
M129T95 129.067 95.252 1.590 1.594 165.272 128.113 39.328 7.446 4.202 2.071 0.001 0.069
M175T288 175.088 287.658 4.790 1.703 19.214 9.525 8.643 2.264 2.223 1.153 0.001 0.069
M133T130 133.032 130.331 2.170 1.923 301.320 46.700 223.018 39.897 1.351 0.434 0.001 0.073
M91T777 90.508 777.121 12.950 2.053 1.070 0.981 6.227 3.665 0.172 -2.541 0.001 0.073
M126T41 125.987 40.955 0.680 1.791 0.576 0.759 5.118 4.279 0.113 -3.151 0.001 0.073
M225T496 225.111 496.480 8.270 2.116 295.192 63.947 154.382 65.598 1.912 0.935 0.001 0.073
M120T36 119.958 35.659 0.590 2.269 2.158 1.591 7.713 2.671 0.280 -1.838 0.001 0.073
M294T465 294.083 464.889 7.750 2.025 38.905 45.004 209.008 119.679 0.186 -2.425 0.001 0.073
M148T89 148.080 88.963 1.480 1.325 1.240 1.098 0.399 0.112 3.104 1.634 0.002 0.082
M98T36 97.970 35.561 0.590 2.150 0.428 0.169 1.015 0.343 0.421 -1.246 0.002 0.082
M552T744 551.832 744.350 12.410 1.650 24.141 43.586 408.442 418.334 0.059 -4.081 0.002 0.082
M262T94 262.093 94.352 1.570 2.067 60.976 19.888 22.376 17.022 2.725 1.446 0.002 0.082
M161T69 160.972 68.917 1.150 2.197 0.940 0.893 3.299 1.097 0.285 -1.810 0.002 0.082
M120T34 119.976 34.085 0.570 1.672 1.258 1.164 7.733 6.829 0.163 -2.620 0.002 0.082
M566T827_2 566.333 826.903 13.780 1.933 470.152 213.664 813.587 155.997 0.578 -0.791 0.002 0.089
M146T528 146.060 527.989 8.800 1.798 204.765 66.864 108.667 51.714 1.884 0.914 0.002 0.089
M219T146 219.099 145.518 2.430 1.747 7.796 3.127 15.599 6.964 0.500 -1.001 0.002 0.089
M243T156 242.984 155.549 2.590 1.835 148.559 68.286 313.726 131.104 0.474 -1.079 0.002 0.089
M289T419_2 289.127 419.090 6.980 1.822 0.390 0.146 0.866 0.412 0.451 -1.149 0.002 0.089
M319T140 319.152 139.877 2.330 1.889 1932.555 963.784 4144.530 1598.515 0.466 -1.101 0.002 0.089
M384T216 384.078 215.625 3.590 2.109 26.816 6.976 11.885 6.814 2.256 1.174 0.002 0.089
M174T376 174.113 376.325 6.270 2.035 6.340 2.326 2.684 0.839 2.362 1.240 0.002 0.089
M149T540 149.022 539.705 9.000 1.437 2.982 2.131 35.451 43.343 0.084 -3.572 0.003 0.094
M102T90 102.056 90.237 1.500 2.086 61.105 6.943 48.227 4.835 1.267 0.341 0.003 0.094
M143T102 143.082 101.741 1.700 1.833 51.975 12.799 34.593 6.892 1.502 0.587 0.003 0.094
M149T92 149.126 91.800 1.530 1.908 1.916 0.101 2.134 0.148 0.898 -0.155 0.003 0.094
M199T205 198.973 205.100 3.420 1.646 89.475 29.855 136.598 39.309 0.655 -0.610 0.003 0.094
M113T91_1 113.001 90.674 1.510 1.674 26.277 13.643 11.268 4.460 2.332 1.222 0.003 0.094
M157T69 156.967 68.920 1.150 2.043 1.849 1.699 7.129 3.465 0.259 -1.947 0.003 0.094
M417T148 417.015 148.041 2.470 1.892 32.009 16.424 102.176 58.383 0.313 -1.675 0.003 0.094
M225T114 224.965 113.724 1.900 2.106 11.411 6.043 29.778 10.920 0.383 -1.384 0.003 0.094
M463T664 463.272 664.271 11.070 1.883 273.037 186.268 668.185 276.479 0.409 -1.291 0.003 0.094
M146T126 146.028 126.295 2.100 1.910 98.860 83.374 264.121 105.689 0.374 -1.418 0.003 0.094
M483T831 483.327 830.947 13.850 1.820 341.665 134.865 171.080 45.647 1.997 0.998 0.004 0.098
M104T130 104.054 130.323 2.170 1.713 0.971 0.282 0.635 0.134 1.528 0.612 0.004 0.098
M256T808 256.228 807.822 13.460 1.832 0.925 0.368 1.450 0.249 0.638 -0.649 0.004 0.098
M159T703 158.987 703.482 11.720 1.424 1.587 1.299 0.492 0.170 3.227 1.690 0.004 0.098
M203T288 203.082 287.531 4.790 1.590 28.118 13.631 14.562 2.865 1.931 0.949 0.004 0.098
M159T728_1 158.961 728.274 12.140 1.455 1.060 0.313 0.687 0.340 1.544 0.627 0.004 0.098
M223T94 223.094 93.687 1.560 2.027 15.197 6.511 26.195 4.001 0.580 -0.786 0.004 0.098
M126T327 126.055 327.460 5.460 2.020 9.875 9.786 27.870 8.257 0.354 -1.497 0.004 0.098
M187T148 187.072 147.583 2.460 1.910 0.608 0.325 1.618 0.784 0.376 -1.413 0.004 0.098
M197T441 197.129 441.228 7.350 1.932 20.959 4.535 10.650 6.963 1.968 0.977 0.004 0.098
M981T512_2 980.988 512.319 8.540 1.949 13.017 8.790 39.866 19.811 0.327 -1.615 0.004 0.098
M391T668 391.212 668.359 11.140 2.051 37.766 11.221 14.747 11.483 2.561 1.357 0.004 0.098
M401T216 401.030 216.047 3.600 1.928 37.707 15.830 11.793 12.432 3.197 1.677 0.004 0.098
M276T312 276.139 311.931 5.200 1.335 501.914 481.936 118.563 142.906 4.233 2.082 0.004 0.098
M494T819 494.325 818.979 13.650 1.845 82.624 12.878 61.070 13.211 1.353 0.436 0.005 0.110
M547T835_1 547.352 835.239 13.920 1.702 850.716 109.490 1134.699 269.401 0.750 -0.416 0.005 0.110
M144T100 144.066 100.293 1.670 1.566 1.781 0.668 1.090 0.314 1.634 0.709 0.005 0.110
M175T104 175.072 103.922 1.730 1.734 12.061 6.518 24.587 10.332 0.491 -1.028 0.005 0.110
M245T37 244.886 36.812 0.610 1.878 0.817 0.189 0.520 0.152 1.571 0.652 0.005 0.110
M175T196 175.088 196.118 3.270 1.631 37.390 19.882 16.750 4.657 2.232 1.159 0.005 0.110
M298T357 298.129 357.450 5.960 1.859 802.481 357.578 302.360 197.096 2.654 1.408 0.005 0.110
M175T141 175.109 141.007 2.350 1.681 4.776 5.452 20.578 15.666 0.232 -2.107 0.005 0.110
M172T73 171.960 73.263 1.220 2.041 1.020 0.479 2.185 0.686 0.467 -1.099 0.005 0.110
M252T555 252.158 554.784 9.250 1.866 1.889 0.786 3.222 0.792 0.586 -0.770 0.005 0.110
M131T45 131.086 45.456 0.760 1.378 26.261 28.942 2.947 2.948 8.912 3.156 0.005 0.110
M162T91 162.113 91.326 1.520 1.803 29093.141 4650.327 43870.026 13023.755 0.663 -0.593 0.007 0.110
M294T251 294.156 250.668 4.180 1.730 101392.410 81376.644 198545.008 35649.045 0.511 -0.970 0.007 0.110
M150T147 150.059 146.614 2.440 1.608 36.967 4.317 32.029 2.780 1.154 0.207 0.007 0.110
M538T824 538.390 823.767 13.730 1.884 126.409 45.111 217.696 61.887 0.581 -0.784 0.007 0.110
M337T103 337.161 102.940 1.720 1.756 788.860 291.708 1440.446 556.095 0.548 -0.869 0.007 0.110
M155T479 154.991 479.438 7.990 1.446 1.342 1.299 0.227 0.124 5.921 2.566 0.007 0.110
M104T831 104.107 830.633 13.840 1.811 145.369 96.993 26.818 20.495 5.420 2.438 0.007 0.110
M100T819 99.514 819.407 13.660 1.546 1.652 0.482 1.004 0.560 1.645 0.719 0.007 0.110
M220T89 220.082 88.870 1.480 1.692 158.049 38.481 213.534 39.012 0.740 -0.434 0.007 0.110
M166T171 166.059 171.198 2.850 1.659 88.978 27.069 51.646 26.547 1.723 0.785 0.007 0.110
M210T67 210.025 67.129 1.120 1.838 1.094 0.634 2.303 0.848 0.475 -1.073 0.007 0.110
M333T754 332.675 753.614 12.560 1.855 1509.470 1680.152 5959.662 3548.237 0.253 -1.981 0.007 0.110
M95T202 95.050 201.780 3.360 1.928 32.851 8.994 16.700 9.008 1.967 0.976 0.007 0.110
M320T97 320.149 97.366 1.620 1.993 391.696 138.486 842.951 319.896 0.465 -1.106 0.007 0.110
M224T145 224.129 145.472 2.420 1.859 3.357 0.727 4.844 1.056 0.693 -0.529 0.007 0.110
M175T134 175.073 134.329 2.240 1.727 97.826 48.437 191.652 78.464 0.510 -0.970 0.007 0.110
M147T103 147.114 103.031 1.720 1.631 10.011 5.776 3.952 1.675 2.533 1.341 0.007 0.110
M190T178 190.108 178.196 2.970 1.682 10.203 3.225 6.451 1.599 1.582 0.661 0.007 0.110
M164T38 163.941 38.404 0.640 1.837 0.533 0.411 1.313 0.547 0.405 -1.302 0.007 0.110
M291T331 291.099 330.667 5.510 1.496 2620.508 2412.702 437.352 376.037 5.992 2.583 0.007 0.110
M230T102 230.103 102.237 1.700 2.190 10.885 4.378 27.997 9.738 0.389 -1.363 0.007 0.110
M294T326 294.156 325.897 5.430 1.708 302.024 118.582 457.502 89.496 0.660 -0.599 0.007 0.110
M176T227 176.067 226.774 3.780 1.912 3.110 1.679 5.822 1.329 0.534 -0.905 0.007 0.110
M202T668 202.218 668.037 11.130 1.541 1.140 0.912 4.306 3.699 0.265 -1.917 0.007 0.110
M273T69 272.882 68.843 1.150 1.499 12.148 10.109 2.929 1.973 4.147 2.052 0.007 0.110
M276T102 276.120 101.630 1.690 1.670 116.490 33.781 180.919 57.808 0.644 -0.635 0.009 0.113
M546T724_1 546.351 724.082 12.070 1.878 19667.105 17103.642 56772.085 26509.343 0.346 -1.529 0.009 0.113
M149T613 149.022 612.505 10.210 1.721 2.281 0.516 2.945 0.348 0.775 -0.368 0.009 0.113
M703T82_2 702.874 81.694 1.360 1.341 2.110 1.998 0.519 0.552 4.066 2.023 0.009 0.113
M522T827 522.343 826.845 13.780 1.621 159.657 38.793 109.502 35.399 1.458 0.544 0.009 0.113
M344T156 344.135 155.549 2.590 1.709 11.918 3.696 7.422 2.051 1.606 0.683 0.009 0.113
M190T825 190.049 824.694 13.740 1.818 0.758 0.516 1.705 0.667 0.445 -1.169 0.009 0.113
M84T76 84.082 76.204 1.270 1.726 295.346 39.362 230.430 49.082 1.282 0.358 0.009 0.113
M115T69 114.972 68.505 1.140 1.919 1.208 0.738 3.475 1.736 0.348 -1.525 0.009 0.113
M483T819_1 483.324 819.273 13.650 1.741 101.999 34.276 57.541 22.236 1.773 0.826 0.009 0.113
M367T338 367.152 338.398 5.640 1.627 19.611 10.053 36.663 15.318 0.535 -0.903 0.009 0.113
M460T806_1 460.260 805.932 13.430 1.767 2.004 0.649 0.975 0.694 2.056 1.040 0.009 0.113
M292T347 292.119 346.672 5.780 1.832 675.265 133.772 474.636 106.953 1.423 0.509 0.009 0.113
M114T536 113.965 535.819 8.930 1.260 1.381 1.898 12.467 17.456 0.111 -3.175 0.009 0.113
M186T146 186.114 145.870 2.430 1.739 3.234 1.243 1.764 0.461 1.833 0.874 0.009 0.113
M100T542 100.077 542.087 9.030 1.278 3.082 3.254 0.646 0.884 4.774 2.255 0.009 0.113
M257T808 257.232 807.519 13.460 1.667 0.641 0.329 1.200 0.474 0.535 -0.904 0.009 0.113
M199T374 199.009 374.113 6.240 1.762 19.214 5.649 9.295 7.411 2.067 1.048 0.009 0.113
M91T503 91.055 502.834 8.380 1.610 35.849 8.165 77.574 46.542 0.462 -1.114 0.009 0.113
M161T216 161.013 216.168 3.600 1.547 5.498 1.681 3.497 1.481 1.572 0.653 0.009 0.113
M309T317 309.139 316.692 5.280 1.712 570.769 244.495 260.086 163.095 2.195 1.134 0.009 0.113
M245T107 244.982 106.945 1.780 1.779 1.933 0.279 2.523 0.478 0.766 -0.384 0.009 0.113
M198T189 198.088 189.214 3.150 1.642 53.726 39.205 107.967 40.148 0.498 -1.007 0.009 0.113
M135T806 135.081 806.110 13.440 1.639 1.189 0.541 2.396 1.188 0.496 -1.011 0.009 0.113
M222T337 222.081 337.080 5.620 1.049 4.208 9.716 21.641 32.676 0.194 -2.362 0.009 0.113
M182T171 182.082 170.988 2.850 1.816 323.985 44.200 248.824 49.656 1.302 0.381 0.011 0.123
M147T76 147.114 76.210 1.270 1.770 143.052 17.861 109.517 25.911 1.306 0.385 0.011 0.123
M118T144 118.087 144.280 2.400 1.532 8.497 0.785 11.060 3.005 0.768 -0.380 0.011 0.123
M569T830 569.339 830.046 13.830 1.707 659.264 384.265 1396.914 631.167 0.472 -1.083 0.011 0.123
M117T230 116.967 229.961 3.830 1.566 1.145 0.529 1.849 0.581 0.619 -0.692 0.011 0.123
M234T91 234.098 91.057 1.520 1.360 36798.530 14032.776 51671.417 14293.012 0.712 -0.490 0.011 0.123
M160T144 160.134 144.009 2.400 1.694 160.230 55.441 96.103 24.519 1.667 0.738 0.011 0.123
M219T104 219.099 103.516 1.730 1.639 8.966 1.518 13.594 4.827 0.660 -0.600 0.011 0.123
M518T806_1 518.317 805.768 13.430 1.745 21.183 4.237 15.749 2.586 1.345 0.428 0.011 0.123
M124T202 124.048 201.663 3.360 1.813 37.867 5.234 29.812 4.729 1.270 0.345 0.011 0.123
M135T82 135.004 81.716 1.360 1.713 6.554 2.679 10.357 2.496 0.633 -0.660 0.011 0.123
M278T129 278.125 128.757 2.150 1.601 651.653 279.718 986.105 215.812 0.661 -0.598 0.011 0.123
M314T154_1 314.108 154.154 2.570 1.477 6309.660 1441.787 8138.110 1693.495 0.775 -0.367 0.011 0.123
M317T147 317.017 146.511 2.440 1.856 5450.731 1558.176 9254.215 2947.084 0.589 -0.764 0.011 0.123
M125T378 125.108 377.705 6.300 1.552 42.331 12.751 55.518 6.436 0.762 -0.391 0.011 0.123
M115T427 115.096 426.872 7.110 1.793 0.440 0.176 0.943 0.440 0.466 -1.101 0.011 0.123
M100T72 100.012 71.934 1.200 1.391 6.212 8.291 241.292 328.162 0.026 -5.279 0.011 0.123
M291T99 291.069 99.391 1.660 1.864 309.095 146.090 632.394 237.942 0.489 -1.033 0.011 0.123
M197T134 197.054 133.952 2.230 1.663 60.251 33.981 129.990 64.962 0.464 -1.109 0.011 0.123
M219T821 219.175 821.006 13.680 1.191 0.566 0.369 1.235 1.057 0.458 -1.126 0.011 0.123
M203T198 203.104 197.669 3.290 1.443 22.393 18.333 46.598 24.571 0.481 -1.057 0.011 0.123
M202T290 202.120 289.961 4.830 1.913 0.864 0.271 7.372 5.560 0.117 -3.094 0.011 0.123
M145T32 145.123 32.494 0.540 1.630 0.194 0.258 0.552 0.273 0.351 -1.509 0.011 0.123
M185T156 184.975 155.673 2.590 1.632 1.601 0.786 2.626 0.716 0.610 -0.714 0.011 0.123
M144T697 144.139 696.802 11.610 1.834 0.460 0.273 1.434 0.845 0.320 -1.642 0.013 0.133
M309T76_1 309.167 76.232 1.270 1.690 620.347 211.168 876.574 126.026 0.708 -0.499 0.015 0.133
M161T104 161.138 103.622 1.730 1.582 788.795 222.744 464.987 281.376 1.696 0.762 0.015 0.133
M184T757 184.074 757.332 12.620 1.954 61.922 35.643 295.175 187.402 0.210 -2.253 0.015 0.133
M185T378 185.129 377.783 6.300 1.725 1291.399 150.982 1494.522 117.470 0.864 -0.211 0.015 0.133
M582T457 582.295 456.952 7.620 1.523 103.155 162.137 506.403 462.121 0.204 -2.296 0.015 0.133
M246T103_2 246.110 103.238 1.720 1.470 3.693 1.931 1.886 0.833 1.958 0.970 0.015 0.133
M190T663 190.087 662.619 11.040 1.752 41.009 13.239 23.250 9.394 1.764 0.819 0.015 0.133
M130T663 130.065 662.678 11.040 1.754 33.484 10.721 19.867 5.979 1.685 0.753 0.015 0.133
M339T326 339.157 326.104 5.440 1.595 48697.880 20987.551 77386.454 23013.452 0.629 -0.668 0.015 0.133
M759T803 758.565 802.915 13.380 1.448 0.586 0.084 1.731 1.512 0.339 -1.562 0.015 0.133
M170T831 170.097 830.925 13.850 1.785 0.408 0.196 0.725 0.211 0.562 -0.831 0.015 0.133
M91T743 90.508 743.484 12.390 1.653 4.259 2.064 1.894 1.100 2.249 1.169 0.015 0.133
M171T194 171.149 193.524 3.230 1.763 1.096 0.673 6.626 5.403 0.165 -2.595 0.015 0.133
M158T360 158.082 360.104 6.000 1.630 277.620 51.633 215.293 37.674 1.289 0.367 0.015 0.133
M224T393 224.076 393.325 6.560 1.539 21.897 34.587 102.263 89.483 0.214 -2.224 0.015 0.133
M371T32 371.105 32.488 0.540 1.646 1.173 0.429 1.776 0.452 0.660 -0.599 0.015 0.133
M144T746 143.982 745.623 12.430 1.583 2.683 0.518 1.915 0.677 1.401 0.487 0.015 0.133
M279T154 278.574 154.068 2.570 1.818 31.834 24.087 92.940 50.359 0.343 -1.546 0.015 0.133
M180T38 180.035 38.237 0.640 1.839 6.120 4.456 36.235 27.193 0.169 -2.566 0.015 0.133
M145T326 145.070 326.158 5.440 1.288 40.378 19.998 61.611 23.651 0.655 -0.610 0.015 0.133
M175T465 175.035 464.874 7.750 1.678 49.943 22.318 93.244 38.711 0.536 -0.901 0.015 0.133
M359T103 359.144 102.795 1.710 1.493 248.054 114.944 448.580 218.472 0.553 -0.855 0.015 0.133
M165T750 165.165 749.949 12.500 1.475 0.239 0.193 0.583 0.387 0.410 -1.288 0.015 0.133
M319T744 318.664 743.670 12.390 1.481 131.058 67.490 482.456 444.172 0.272 -1.880 0.015 0.133
M176T526 176.072 525.644 8.760 1.396 19.137 8.337 28.971 9.873 0.661 -0.598 0.015 0.133
M714T416_3 713.929 416.055 6.930 1.299 82.389 21.509 60.253 23.371 1.367 0.451 0.015 0.133
M367T216 367.065 216.329 3.610 1.536 480.885 262.650 208.761 145.878 2.304 1.204 0.015 0.133
M186T511 186.114 510.725 8.510 1.422 43.333 21.129 21.343 17.739 2.030 1.022 0.015 0.133
M276T251 276.146 250.790 4.180 1.390 14264.017 6260.374 20053.989 3891.886 0.711 -0.492 0.019 0.144
M524T756_1 524.359 756.408 12.610 1.495 86.635 82.303 219.312 139.738 0.395 -1.340 0.019 0.144
M148T90 148.061 90.408 1.510 1.456 91.252 14.703 74.772 13.779 1.220 0.287 0.019 0.144
M110T830 110.021 829.814 13.830 1.402 2.195 2.780 16.271 19.195 0.135 -2.890 0.019 0.144
M516T819 516.307 819.106 13.650 1.719 505.467 156.505 306.789 101.155 1.648 0.720 0.019 0.144
M166T202 166.059 201.729 3.360 1.575 54.805 9.273 42.794 9.416 1.281 0.357 0.019 0.144
M542T807 542.319 807.168 13.450 1.669 132.452 54.860 67.551 31.980 1.961 0.971 0.019 0.144
M248T101 248.114 100.829 1.680 1.368 321.277 119.789 420.468 39.873 0.764 -0.388 0.019 0.144
M230T251 230.140 250.886 4.180 1.611 795.045 394.648 1320.871 396.562 0.602 -0.732 0.019 0.144
M124T793 124.088 793.237 13.220 1.626 1.214 0.798 0.387 0.199 3.135 1.649 0.019 0.144
M367T165 367.027 164.870 2.750 1.334 81.020 54.770 37.152 18.131 2.181 1.125 0.019 0.144
M312T419 312.203 418.994 6.980 1.712 0.814 0.246 1.194 0.277 0.682 -0.552 0.019 0.144
M150T73 149.954 73.256 1.220 1.724 0.879 0.288 0.454 0.288 1.937 0.954 0.019 0.144
M197T104 197.054 103.919 1.730 1.419 53.600 27.417 88.738 36.265 0.604 -0.727 0.019 0.144
M104T345 104.059 345.028 5.750 1.544 4.112 2.025 1.874 1.435 2.195 1.134 0.019 0.144
M337T140 337.161 139.709 2.330 1.383 48.259 19.161 85.548 47.746 0.564 -0.826 0.019 0.144
M160T495 160.098 494.885 8.250 1.169 139.764 66.466 212.439 101.476 0.658 -0.604 0.019 0.144
M173T100 172.957 99.797 1.660 1.316 1.360 0.674 2.002 0.587 0.679 -0.558 0.019 0.144
M157T111 157.019 111.261 1.850 1.769 1.828 0.270 2.212 0.221 0.826 -0.275 0.019 0.144
M166T327 166.049 327.421 5.460 1.221 13.411 7.752 21.502 10.030 0.624 -0.681 0.019 0.144
M160T480 160.098 479.832 8.000 1.301 80.499 30.440 122.524 53.932 0.657 -0.606 0.019 0.144
M163T150 163.124 149.595 2.490 1.180 0.602 0.295 0.931 0.456 0.647 -0.629 0.019 0.144
M181T39 181.034 38.504 0.640 1.016 0.480 0.407 0.213 0.293 2.250 1.170 0.019 0.144
M323T629 323.184 629.492 10.490 1.184 64.228 38.656 33.989 27.550 1.890 0.918 0.019 0.144
M219T165 218.984 164.891 2.750 1.329 10.789 9.516 3.150 3.416 3.426 1.776 0.019 0.144
M737T416 737.240 415.599 6.930 1.150 1133.798 535.546 685.504 513.884 1.654 0.726 0.019 0.144
M152T277 152.066 277.114 4.620 1.667 7.431 2.088 4.538 2.034 1.638 0.712 0.019 0.144
M325T189 325.016 189.026 3.150 1.418 252.280 127.392 134.522 69.353 1.875 0.907 0.019 0.144
M257T211 257.015 211.014 3.520 1.491 16.339 11.202 31.230 14.029 0.523 -0.935 0.019 0.144
M148T583 148.076 583.157 9.720 1.958 11.884 3.437 4.109 5.031 2.892 1.532 0.019 0.144
M307T217 307.073 217.114 3.620 1.152 24.682 8.401 215.258 337.336 0.115 -3.124 0.019 0.144
M160T104 160.133 103.596 1.730 1.507 1717.971 501.226 1211.166 282.269 1.418 0.504 0.023 0.160
M520T837_1 520.327 836.729 13.950 1.216 98.365 99.730 27.042 34.330 3.638 1.863 0.023 0.160
M294T225 294.156 224.753 3.750 1.374 47375.147 19181.404 69069.682 21809.757 0.686 -0.544 0.023 0.160
M262T216 262.014 215.944 3.600 1.453 34293.645 10542.036 23739.864 7092.753 1.445 0.531 0.023 0.160
M274T91 274.091 91.393 1.520 1.333 6806.423 2016.512 8764.527 1769.092 0.777 -0.365 0.023 0.160
M170T148 170.040 147.905 2.470 1.629 59.426 7.441 70.774 9.475 0.840 -0.252 0.023 0.160
M569T824 569.336 823.721 13.730 1.400 930.488 420.620 1572.073 749.826 0.592 -0.757 0.023 0.160
M204T202 204.064 202.018 3.370 1.603 250.979 34.072 196.219 49.378 1.279 0.355 0.023 0.160
M319T103 319.152 103.105 1.720 1.452 460.222 269.840 786.171 301.820 0.585 -0.773 0.023 0.160
M269T819 269.213 819.210 13.650 1.047 0.973 0.496 0.629 0.385 1.547 0.629 0.023 0.160
M154T283 154.070 282.536 4.710 1.499 78.257 15.852 58.570 17.134 1.336 0.418 0.023 0.160
M234T146 234.134 145.631 2.430 1.159 184.405 44.397 221.521 41.480 0.832 -0.265 0.023 0.160
M248T225 248.150 224.875 3.750 1.540 4842.749 1972.511 7958.964 3053.016 0.608 -0.717 0.023 0.160
M141T103 141.067 102.974 1.720 1.254 24.991 18.111 11.593 5.932 2.156 1.108 0.023 0.160
M547T819 547.368 819.253 13.650 1.328 190.343 109.583 352.458 205.831 0.540 -0.889 0.023 0.160
M302T755 302.270 754.760 12.580 1.333 2.833 2.166 1.028 1.035 2.756 1.463 0.023 0.160
M144T178 144.103 178.231 2.970 1.492 6.712 1.689 5.038 0.922 1.332 0.414 0.023 0.160
M121T105 121.095 105.239 1.750 1.394 1.186 0.254 1.409 0.115 0.842 -0.248 0.023 0.160
M144T774 143.982 773.816 12.900 1.310 0.842 0.191 0.668 0.147 1.260 0.333 0.023 0.160
M295T154_2 295.123 153.666 2.560 1.300 3806.239 1185.244 2417.920 1635.242 1.574 0.655 0.023 0.160
M358T779 358.160 778.750 12.980 1.777 40.595 24.434 126.355 78.848 0.321 -1.638 0.023 0.160
M310T103 310.150 103.483 1.720 1.421 58.257 20.097 107.544 62.871 0.542 -0.884 0.023 0.160
M343T215 343.051 215.226 3.590 1.539 9.581 4.149 14.687 4.043 0.652 -0.616 0.023 0.160
M429T99 429.092 98.850 1.650 1.626 18.660 4.732 29.246 10.583 0.638 -0.648 0.023 0.160
M290T156_1 289.982 155.533 2.590 1.323 179.340 71.547 268.098 104.524 0.669 -0.580 0.023 0.160
M273T156 272.973 155.731 2.600 1.616 12.186 2.755 16.436 3.654 0.741 -0.432 0.023 0.160
M232T203 232.028 203.467 3.390 1.641 238.188 198.031 473.011 130.246 0.504 -0.990 0.023 0.160
M183T323 183.114 322.916 5.380 1.528 2.808 1.249 4.542 1.589 0.618 -0.694 0.023 0.160
M515T216 515.033 216.096 3.600 1.582 110.030 65.902 40.977 30.199 2.685 1.425 0.023 0.160
M215T257 215.140 257.313 4.290 1.775 1.185 0.566 2.356 0.946 0.503 -0.991 0.023 0.160
M149T318 149.022 318.499 5.310 1.403 0.189 0.178 0.901 0.962 0.210 -2.252 0.029 0.177
M196T818 196.016 818.495 13.640 1.655 2.692 2.034 0.544 0.412 4.947 2.307 0.029 0.177
M185T773 184.987 773.208 12.890 1.512 4.342 2.366 2.025 1.065 2.144 1.101 0.029 0.177
M302T82 302.146 81.695 1.360 1.655 0.406 0.216 0.726 0.249 0.559 -0.840 0.029 0.177
M279T808 279.233 808.460 13.470 1.462 13.851 5.589 8.600 2.606 1.611 0.688 0.029 0.177
M128T204 128.064 203.777 3.400 1.351 2.276 1.802 5.172 3.671 0.440 -1.184 0.029 0.177
M247T334 247.130 333.793 5.560 1.611 171.635 17.413 204.200 31.330 0.841 -0.251 0.029 0.177
M202T364 202.109 363.626 6.060 1.526 507.098 66.396 597.479 81.781 0.849 -0.237 0.029 0.177
M118T493 118.066 493.273 8.220 1.360 1.828 1.236 3.534 2.016 0.517 -0.951 0.029 0.177
M108T37 107.951 37.123 0.620 1.392 2.234 0.873 1.319 0.805 1.694 0.760 0.029 0.177
M385T103 385.038 103.197 1.720 1.571 1206.937 402.052 1722.086 402.131 0.701 -0.513 0.029 0.177
M498T670 498.288 669.708 11.160 1.448 14.097 6.361 25.826 13.733 0.546 -0.873 0.029 0.177
M120T501 120.025 501.310 8.360 1.260 0.623 0.407 1.870 1.923 0.333 -1.586 0.029 0.177
M223T639 223.097 639.038 10.650 1.429 39.881 6.452 27.012 15.714 1.476 0.562 0.029 0.177
M174T342 174.056 341.555 5.690 1.534 15.060 4.324 7.978 7.119 1.888 0.917 0.029 0.177
M191T178 191.111 178.231 2.970 1.445 89.165 20.653 67.770 16.029 1.316 0.396 0.029 0.177
M272T65 271.874 64.754 1.080 1.537 1.800 0.552 2.405 0.387 0.748 -0.418 0.029 0.177
M416T806 416.217 805.721 13.430 1.381 1.522 0.850 2.335 0.621 0.652 -0.618 0.029 0.177
M264T646 264.159 646.393 10.770 1.260 0.396 0.368 0.737 0.344 0.537 -0.896 0.029 0.177
M277T154 277.095 154.465 2.570 1.627 55.025 19.453 78.170 13.609 0.704 -0.507 0.029 0.177
M113T734 112.519 734.408 12.240 1.173 1.372 1.449 0.388 0.459 3.540 1.824 0.029 0.177
M211T460 211.145 459.861 7.660 1.509 107.846 25.223 72.373 33.848 1.490 0.575 0.029 0.177
M296T818 296.141 817.798 13.630 1.521 42.858 21.608 18.422 17.653 2.326 1.218 0.029 0.177
M266T327 266.052 327.466 5.460 1.572 114.583 60.981 211.263 90.533 0.542 -0.883 0.029 0.177
M412T492 412.209 491.885 8.200 1.331 22.041 8.154 14.677 5.756 1.502 0.587 0.029 0.177
M275T112 275.025 111.810 1.860 1.545 819.928 471.911 1356.549 366.695 0.604 -0.726 0.029 0.177
M516T499 516.117 498.790 8.310 1.569 321.620 109.540 178.071 115.608 1.806 0.853 0.029 0.177
M285T66 284.905 65.764 1.100 1.025 1.896 0.509 1.568 0.329 1.209 0.273 0.029 0.177
M185T111 185.025 111.135 1.850 1.497 1.802 0.353 2.287 0.464 0.788 -0.344 0.029 0.177
M801T572_9 801.440 572.190 9.540 1.413 104.023 73.164 185.762 74.943 0.560 -0.837 0.029 0.177
M97T107 97.012 106.850 1.780 1.534 1.196 0.533 1.793 0.407 0.667 -0.584 0.029 0.177
M312T154 312.112 154.125 2.570 1.401 9.261 1.986 11.675 2.465 0.793 -0.334 0.037 0.191
M130T76 130.087 76.210 1.270 1.374 31.391 4.547 26.433 4.784 1.188 0.248 0.037 0.191
M278T103 278.125 103.314 1.720 1.259 122.561 42.401 160.459 36.204 0.764 -0.389 0.037 0.191
M83T732 83.061 732.316 12.210 1.164 0.885 0.911 3.466 4.390 0.255 -1.969 0.037 0.191
M126T104_1 126.067 103.679 1.730 1.387 365.902 31.710 333.514 27.731 1.097 0.134 0.037 0.191
M197T86 197.102 85.808 1.430 1.380 98.546 49.621 52.495 32.474 1.877 0.909 0.037 0.191
M276T154 276.091 154.473 2.570 1.407 6336.712 1359.491 7901.476 1500.091 0.802 -0.318 0.037 0.191
M543T827_1 543.320 826.836 13.780 1.520 176.820 54.170 113.101 49.351 1.563 0.645 0.037 0.191
M112T88 112.158 87.889 1.460 1.386 2.079 0.205 2.393 0.375 0.869 -0.203 0.037 0.191
M268T90 268.103 90.265 1.500 1.591 2133.383 710.875 3017.309 631.245 0.707 -0.500 0.037 0.191
M244T146 244.119 145.558 2.430 1.316 2245.003 659.744 2880.721 592.767 0.779 -0.360 0.037 0.191
M120T60 120.024 60.198 1.000 1.177 0.562 0.189 0.400 0.179 1.406 0.491 0.037 0.191
M279T639 279.158 639.229 10.650 1.371 1.311 1.090 4.183 3.897 0.313 -1.675 0.037 0.191
M222T216 221.984 215.546 3.590 1.407 29.691 9.554 39.994 9.141 0.742 -0.430 0.037 0.191
M242T36 241.884 35.570 0.590 1.344 0.639 0.443 1.387 0.969 0.461 -1.117 0.037 0.191
M191T816 191.144 816.207 13.600 1.572 8.097 3.972 14.682 6.323 0.551 -0.859 0.037 0.191
M173T37 172.978 36.873 0.610 1.320 0.702 0.455 1.124 0.362 0.625 -0.679 0.037 0.191
M250T146 250.130 146.482 2.440 1.263 155.608 84.777 82.448 64.306 1.887 0.916 0.037 0.191
M118T544 118.066 543.881 9.060 1.387 7.703 4.519 3.853 1.695 1.999 0.999 0.037 0.191
M146T36 145.955 35.561 0.590 1.269 1.849 0.358 2.164 0.285 0.854 -0.227 0.037 0.191
M86T90 86.061 90.237 1.500 1.382 1.417 0.634 0.834 0.396 1.698 0.764 0.037 0.191
M93T345 93.071 345.286 5.750 1.474 1.338 0.755 2.106 0.497 0.636 -0.654 0.037 0.191
M283T69 282.910 68.680 1.140 1.795 1.007 0.684 3.231 1.985 0.312 -1.682 0.037 0.191
M280T201 280.031 200.541 3.340 1.302 162.314 50.335 118.747 33.770 1.367 0.451 0.037 0.191
M114T40 114.056 39.612 0.660 1.631 1.249 0.300 2.393 1.230 0.522 -0.938 0.037 0.191
M275T91 275.095 91.428 1.520 1.500 1000.871 268.793 1388.539 381.695 0.721 -0.472 0.037 0.191
M293T827 293.212 826.776 13.780 1.302 18.558 8.244 10.641 7.593 1.744 0.802 0.037 0.191
M236T216 235.999 216.213 3.600 1.476 317.067 99.727 213.318 71.314 1.486 0.572 0.037 0.191
M155T300 155.083 299.913 5.000 1.844 7.135 4.177 1.834 1.035 3.891 1.960 0.037 0.191
M214T206 213.981 205.520 3.430 1.222 13.042 5.222 17.194 3.425 0.759 -0.399 0.037 0.191
M189T146_1 189.088 145.510 2.430 1.453 1.318 0.548 1.932 0.516 0.682 -0.551 0.037 0.191
M166T429 166.087 428.730 7.150 1.410 77.929 17.869 60.389 12.971 1.290 0.368 0.037 0.191
M247T216 247.050 216.077 3.600 1.500 2.277 1.180 1.117 0.588 2.038 1.027 0.037 0.191
M156T591 156.041 590.985 9.850 1.129 2.187 0.799 2.825 0.722 0.774 -0.370 0.037 0.191
M155T70 155.155 70.397 1.170 1.492 7.835 4.311 3.437 2.697 2.280 1.189 0.037 0.191
M153T147 153.057 146.995 2.450 1.207 6.856 3.114 3.675 3.979 1.865 0.900 0.037 0.191
M146T137 146.046 137.134 2.290 1.339 13.389 3.396 16.698 2.963 0.802 -0.319 0.037 0.191
M222T101 222.098 100.894 1.680 1.563 10.370 8.299 68.612 68.589 0.151 -2.726 0.037 0.191
M352T639 352.247 638.686 10.640 1.210 29.299 24.524 12.528 3.081 2.339 1.226 0.037 0.191
M329T155 328.984 155.354 2.590 1.450 9.061 2.905 5.554 3.253 1.631 0.706 0.037 0.191
M520T837_2 520.340 836.743 13.950 1.421 30.763 7.573 40.355 9.782 0.762 -0.392 0.046 0.214
M118T105 118.087 105.212 1.750 1.441 20.262 0.595 21.397 1.352 0.947 -0.079 0.046 0.214
M175T87 175.120 86.784 1.450 1.092 29.248 8.800 22.928 6.513 1.276 0.351 0.046 0.214
M635T82 634.886 81.696 1.360 1.059 4.715 2.255 8.717 7.393 0.541 -0.886 0.046 0.214
M203T111 203.053 111.144 1.850 1.307 292.494 191.832 469.951 157.952 0.622 -0.684 0.046 0.214
M233T216 233.061 215.849 3.600 1.194 138.935 47.054 104.074 25.450 1.335 0.417 0.046 0.214
M313T79 313.036 79.073 1.320 1.455 92230.587 42321.662 132592.424 22562.663 0.696 -0.524 0.046 0.214
M181T838 181.015 838.229 13.970 1.170 1.934 0.061 1.869 0.090 1.035 0.049 0.046 0.214
M258T151 258.110 150.711 2.510 1.366 144.277 15.113 114.883 38.373 1.256 0.329 0.046 0.214
M114T404 114.038 403.608 6.730 1.295 12.902 14.902 1.800 2.018 7.167 2.841 0.046 0.214
M192T80 192.081 80.233 1.340 1.450 209.245 27.976 172.693 36.359 1.212 0.277 0.046 0.214
M151T130 151.063 130.314 2.170 1.177 38.619 10.818 30.442 6.958 1.269 0.343 0.046 0.214
M269T103 269.125 103.067 1.720 1.319 62.444 62.145 132.390 77.455 0.472 -1.084 0.046 0.214
M166T824 165.983 823.953 13.730 1.252 1.413 0.913 3.896 3.833 0.363 -1.464 0.046 0.214
M379T216 379.048 215.849 3.600 1.427 40.786 17.486 24.592 9.173 1.659 0.730 0.046 0.214
M311T95 311.117 95.102 1.590 1.242 92.967 84.816 203.717 150.667 0.456 -1.132 0.046 0.214
M230T104 230.159 103.958 1.730 1.446 10.252 9.466 19.566 6.097 0.524 -0.932 0.046 0.214
M146T508 146.061 508.387 8.470 1.303 55.553 21.493 79.213 26.429 0.701 -0.512 0.046 0.214
M140T69 139.964 68.921 1.150 1.236 0.899 0.752 2.183 1.904 0.412 -1.281 0.046 0.214
M133T89 133.062 88.745 1.480 1.207 24.780 6.622 29.661 3.205 0.835 -0.259 0.046 0.214
M275T818 274.626 817.873 13.630 1.276 765.613 294.776 467.592 323.874 1.637 0.711 0.046 0.214
M253T216 253.020 215.835 3.600 1.547 85.087 27.667 116.497 21.262 0.730 -0.453 0.046 0.214
M508T809 508.389 808.647 13.480 1.379 6.000 2.111 8.507 2.611 0.705 -0.504 0.046 0.214
M204T334 204.124 333.875 5.560 1.452 89.255 76.677 219.627 148.288 0.406 -1.299 0.046 0.214
M200T216 200.002 215.845 3.600 1.293 1.127 0.725 1.973 1.003 0.572 -0.807 0.046 0.214
M303T216 303.041 215.852 3.600 1.314 46.174 15.921 31.595 12.460 1.461 0.547 0.046 0.214
M286T408 285.611 407.834 6.800 1.516 0.573 0.135 0.720 0.101 0.795 -0.331 0.046 0.214
M344T809 344.353 808.616 13.480 1.363 0.698 0.471 1.285 0.649 0.544 -0.879 0.046 0.214
M101T36 100.933 35.561 0.590 1.407 2.190 0.482 2.764 0.571 0.792 -0.336 0.046 0.214
M130T114 129.987 113.964 1.900 1.090 2.005 0.890 2.684 0.794 0.747 -0.421 0.046 0.214
M131T402 131.070 402.433 6.710 1.278 34.461 14.808 46.058 6.653 0.748 -0.418 0.046 0.214
M113T146_2 113.164 146.455 2.440 1.428 3.206 1.127 2.097 0.777 1.529 0.613 0.046 0.214
M252T512 252.158 512.301 8.540 1.436 1.456 0.373 1.059 0.321 1.375 0.460 0.046 0.214
M204T321 204.067 321.388 5.360 1.339 11.560 5.945 5.938 4.755 1.947 0.961 0.046 0.214
mz:质核比;rt:保留时间(以秒为单位);rtmin:保留时间(以分钟为单位);*_VIP:VIP值;mean_*:平均值;sd_*:标准差;fold change:倍性变化;log2(FC):倍性变化的log2值;pvalue:统计学p值;FDR:pvalue校正值
以下为差异待鉴定代谢物统计结果:
图:差异代谢物统计柱状图

代谢物上下调关系

  updownstats.csv